Monsanto and Illummina Reach Key Milestone in Cotton Genome Sequencing
Thursday, September 30th, 2010
The complicated cotton genome is one step closer to having its genetic threads unraveled, thanks to a key research milestone completed and announced today by Monsanto Company (NYSE:MON) and San Diego-based Ilumina Inc. (NASDAQ: ILMN).
Combining Monsanto's knowledge of cotton genomics and Illumina's next generation sequencing technology, a critical landmark has been achieved that could lead to the development of cotton crops with higher yields, better fiber quality, and greater resistance to diseases and pests.
The two companies have completed sequencing a wild Peruvian cotton species, Gossypium raimondii, and will donate their findings to the public. The completion of G. raimondii will aid public and private researchers in their quest to sequence the more elusive genome of domesticated cotton, G. hirsutum.
Domesticated cotton, more commonly known as American Upland cotton, accounts for more than 95 percent of U.S. production. Its genome has proven difficult to sequence and assemble because of its large size as well as the large quantity of repetitive DNA. The cotton genome, at about 2.7 billion nucleotides, is roughly comparable to the human genome at 3.2 billion. Additionally, most organisms-including humans-have two sets of chromosomes. However, domesticated cotton has four sets.
"Imagine you have four puzzles and all of their numerous pieces to put together in order," says Ty Vaughn, Monsanto global cotton technology lead. "On top of that, many of the puzzle pieces are identical. The cotton genome presents the same unique challenge to researchers."
Researchers chose a strategy to study related cotton species that closely represent the more complex domesticated cotton genome. The genetic structure of the G. raimondii species represents one part, and the G. arboretum species, more commonly known as tree cotton, represents the other. Additionally, previous molecular studies have shown that the G. raimondii and G. arboreum genomes have a great deal of similarity in the way that genes are arranged on chromosomes. This could allow the G. raimondii genomic sequence to serve as a base to assemble the even larger and more complicated G. arboreum genome. Having those two sequences together is expected to provide a path toward sequencing and understanding the genome of domesticated cotton.
"Today's announcement will bring everyone closer to assembling the entire cotton genetic puzzle. Sequencing helps cotton breeders and researchers identify which genes are responsible for which characteristics in the crop," says Vaughn. "A high-quality genome map can help us get where we need to go faster and the more detailed that map the faster we can get there."
"The G. raimondii genome sequencing project is a great example of Illumina's commitment to deep collaborations that leverage the quality, throughput and utility of our industry-leading technology," said Michael Thompson, Ph.D., Global Sales Manager of Agrigenomics at Illumina. "We are pleased that this work will enable a thorough understanding of gene content and organization, and lay the ground work for unraveling and harnessing the diversity among the Gossypium genome types. These advancements will ultimately benefit both producers and consumers."
Monsanto and Illumina will deposit the genetic data into the public domain through entry in the GenBank database, hosted by the National Center for Biotechnology Information. Monsanto has a history of donating its genomic knowledge to the public sector. In previous years, the company has made its sequencing of the Arabidopsis genome public and contributed significant genomic data to help complete the rice and corn genomes.
In cotton, Monsanto previously donated 4,000 cotton molecular markers and associated information to Texas AgriLife Research, an agency of the Texas A&M System, in April 2009. These types of public donations should help cotton research continue to move forward.